A platform to view huntingtin exon 1 aggregation flux in the cell reveals divergent influences from chaperones hsp40 and hsp70

AR Ormsby; YM Ramdzan; YF Mok; KD Jovanoski; DM Hatters

Journal article

A platform to view huntingtin exon 1 aggregation flux in the cell reveals divergent influences from chaperones hsp40 and hsp70

AR Ormsby, YM Ramdzan, YF Mok, KD Jovanoski, DM Hatters

Journal of Biological Chemistry | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | Published : 2013

DOI: 10.1074/jbc.M113.486944

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Abstract

Background: How misfolded proteins such as mutant huntingtin aggregate in the cell remains enigmatic. Results:Webuilt a platform to view how aggregation proceeds and assessed the impact of quality control chaperones hsp40 and hsp70. Conclusion: hsp70 enhanced survival of cells with aggregates; hsp40 suppressed aggregation. Significance: We developed a new toolkit to illustrate the impact of protein aggregation on cell biology. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

University of Melbourne Researchers

Yee Foong Mok Author

Danny M Hatters Author

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Grants

Awarded by Hereditary Disease Foundation

Funding Acknowledgements

[ "This work was supported by National Health and Medical Research Council Project Grants 566640, APP1049458, and APP1049459 (to D. M. H.), Australian Research Council Discovery Grant DP120102763, and the Hereditary Disease Foundation, New York.", "Grimwade Fellow supported by the Miegunyah Trust and is currently an Australian Research Council Future Fellow." ]